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Let the Mitochondria answer
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Other techniques

Mitologics expertise extends to mechanistic studies on whole cells by measurement of:

Cell death markers :

  • Cell viability
  • Mitochondrial transmembrane potential
  • Oxygen consumption
  • ADP/ATP ratio
  • Mitochondrial fusion/fission
  • Cytochrome c release
  • Caspases activation
  • Phosphatidylserine exposure
  • Apoptotic genes expression profile
  • Autophagosome formation
A selection of parameters helps to distinguish between Necrosis, Apoptosis, Autophagy, Mitophagy…

Oxidative stress markers:

  • Mitochondrial ROS production
  • Global ROS production
  • GSH/GSSG levels
  • Catalase activity
  • Oxidative stress genes expression profile

Lipogenesis markers :

  • Fatty acid b-oxidation
  • Lipids content detection
  • Glucose uptake
 

Cellular assays are applicable to:

  • Small molecules, Natural extracts, Cell penetrating-peptides, Antibodies
  • Protein knock-down (by siRNA, shRNA)
  • Protein over-expression (by transfection or infection for gene therapy)

Borgne-Sanchez et al., Cell Death Differ 2007 ; Brabant et al., Apoptosis 2009 ; Buron et al., PLoS One 2010